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sinI [2017-8-11 16:0:3]
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sinI [2017-8-11 16:0:3]

antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state
locus
BSU24600
pI
6.33
mw
6.47 kDa
protein length
57 aa Sequence Blast
gene length
171 bp Sequence Blast
function
control of biofilm formation
product
antagonist of SinR
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProt

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]

    • Gene

    Phenotypes of a mutant

  • altered cell death pattern in colonies [Pubmed|23012477]

    • The protein

    Paralogous protein(s)

  • [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA]

    • Structure

  • [PDB|1B0N] (complex [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]-[protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI]) [Pubmed|9799632]

    • Expression and Regulation

    Operons

    genes
    [gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]
    description
    [Pubmed|3125149]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3125149], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, in a portion of cells [Pubmed|11751836], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|1664536], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|7635837,11751836], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [PubMed|1906467,11751836], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

    • regulation

  • repressed during exponential growth ([protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]

    • additional information

  • the mRNA is stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
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    Biological materials

    Mutant

  • GP959 (spc), available in [SW|Jörg Stülke]'s lab
  • DS91 (spc) NCIB3610 derivate, available in [SW|Jörg Stülke]'s lab
  • 1S98 ( ''sinI''::''kan''), [Pubmed|11466285], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
  • GP1663 (''[gene|22BEB39F735DAAF71F1B382CD453A9C2D4CC9FA4|yqhG]-[gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]''), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 20541494

    • Modelling of the [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI]]/[[SinR] switch

  • 21095906

    • Original publications

  • 23430750,23012477,21326214,21815947,3125149,15661000,18047568,11751836,1906467,11751836,7635837,11751836,10547280,15104138,9799632,20923420,8422983,24256735